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KMID : 0604520140400040359
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Journal of the Society of Cosmetic Scientists of Korea
2014 Volume.40 No. 4 p.359 ~ p.364
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Determination of p-Anisic Acid and Methylparaben by Using High Performance Liquid Chromatography
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Kim Il-Hyun
Ryu De-Hun
Kim Young-Soo
Jung Eun-Sun
Park Deok-Hoon
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Abstract
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In this study, we developed a HPLC method for the separation and analysis of methylparaben and p-anisic acid, which are commonly used as a synthetic preservative and natural preservative, respectively. Methylparaben and p-anisic acid have same molecular weight (152.15 g/mol), similar structure and same maximum absorption wavelength (250 nm), thus they showed same retention time (RT) value (13.3 min) in HPLC experiment. We observed that two substances are separated on C18 column after methylparaben was derivatized selectively through the acetylation reaction. Instead, RT of the acetylated methylparaben was moved to 23.9 min from 13.3 min. The average retention time was 23.9±0.1min and peak area values was 5042882±4778. In addition it showed a high linearity in the calibration curve with a correlation coefficient (R2) of 0.9999658. Detection and quantitation limits were 1.47μg/mL and 4.44μg/mL, respectively. In conclusion, the developed method can be useful for separation and analysis of preservatives with similar structure in cosmetic fields.
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KEYWORD
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methylparaben, p-anisic acid, high performance liquid chromatography, Acetylation, preservatives
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